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. 2015 Mar 5;10(3):e0119361. doi: 10.1371/journal.pone.0119361

Fig 6. Tyr-554 phosphorylation of Git1 did not affect its binding to Pix or self-oligomerization.

Fig 6

A, Western blotting of protein expression levels, and tyrosine phosphorylation of all proteins in HEK293T cells expressing FLAG-tagged Git1 proteins together with Myc-tagged Pix. B, Co-immunoprecipitaion of Git1 mutants with βPix using anti-FLAG beads. The figures are representative of two separate experiments. C, Western blotting of HEK293T cells expressing FLAG-tagged Git1 mutants together with YFP-fused wild-type Git1. FLAG-tagged and YFP-tagged Git1 proteins were detected with anti-FLAG M2 and anti-GFP antibodies, respectively. D, Co-immunoprecipitaion experiments using anti-FLAG beads. The figures are representative of two separate experiments.