Table 1.
Primers used in this study
| Primer | ||
| KRAS | Forward | 5’-GGCCTGCTGAAAATGACTGA-3’ |
| Reverse | 5’-GTTGGATCATATTCGTCCAC-3’ | |
| BRAF | Forward | 5’-CCTTTACTTACTACACCTCAG-3’ |
| Reverse | 5’-CATCCACAAAATGGATCCAG-3’ | |
| BAT25 | Forward1 | 5’-TCGCCTCCAAGAATGTAAGT-3’ |
| Reverse | 5’-TCTGCATTTTAACTATGGCTC-3’ | |
| BAT26 | Forward1 | 5’-TGACTACTTTTGACTTCAGCC-3’ |
| Reverse | 5’-AACCATTCAACATTTTTAACCC-3’ | |
| D2S123 | Forward1 | 5’-GAAACAGGATCCTGCCTTTA-3’ |
| Reverse | 5’-GGACTTTCCACCTATGGGAC-3’ | |
| D5S346 | Forward1 | 5’-GACTCACTCTAGTATAAATCGGG-3’ |
| Reverse | 5’-GAGCAGATAAGACAGTATTACTAGTT-3’ | |
| D17S250 | Forward1 | 5’-GGGAAGAATCAAATAGACAAT-3’ |
| Reverse | 5’-GCTGGCCATATATATATTTAAACC-3’ |
1
Forward primers for microsatellite instability (MSI) analysis were fluorescently-labeled with 6-carboxyfluorescein (6-FAM).