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. 2015 Mar 4;17(2):155–166. doi: 10.1016/j.neo.2014.12.003

Figure 1.

Figure 1

NHE1 is associated with EGFR in PDAC and is the major EGFR-driven pHi regulator.

(A) Gene expression profiles of microdissected PDAC and pancreatic tumor, normal, and stellate cell lines using high-density DNA microarray were analyzed with the EGAN program for association nodes in a subset of ion transporter proteins and signal transduction molecules. The length of a connection reflects its strength.

(B) Representative Western blots showing the relative expression of NHE1 (100 and 115 kDa) and EGFR/p(Y1173)-EGFR in the four PDAC cell lines. β-Actin loading controls. See also Figure S1.

(C) EGF stimulates NHE1 activity in the four PDAC cell lines. Error bars, ± SEM, unpaired Student’s t test. *P < .05, **P < .01, and ***P < .001 compared with the control cells for each line (n = 5). Cariporide, 500 nM. See also Figure S2.