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. 2015 Mar 4;17(2):155–166. doi: 10.1016/j.neo.2014.12.003

Figure 3.

Figure 3

Wavelike, reciprocal regulation of EGFR and NHERF1 expression by EGF is maintained by ubiquitin-mediated degradation mechanisms.

(A) NHERF1, EGFR, p(Y1173)-EGFR, and NHE1 protein levels in basal and EGF stimulated (100 ng/ml) conditions in control and wild-type (WT) NHERF1-overexpressing PANC-1 cells. Protein loading normalized to β-actin levels. See also Figure S5.

(B) Graphs showing interdependent modulation of EGFR and NHERF1 expression measured (A) during EGF stimulation in control (left panel) and NHERF10overexpressing (right panel) cells. Error bars, ± SEM (n = 6 experiments).

(C, D) Immunofluorescence images of NHERF1 and EGFR for control (C) and WT-NHERF1 (D)–overexpressing cells. Scale bars, 20μm. See also Figure S6.

(E, F) Graphs showing Mg132-dependent reduction of the EGF-induced modulation of NHERF1 (upper panel) and EGFR (lower panel) expression for control (E) and WT-NHERF1 (F) cells. Error bars, ± SEM (n = 3 experiments).