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. 2015 Mar 4;17(2):215–224. doi: 10.1016/j.neo.2014.12.011

Figure 2.

Figure 2

Phenotypic analysis of NU12 and SK-RC-52 tumors.

Phenotypic analysis of NU12 and SK-RC-52 tumors of mice treated with sunitinib shows necrosis, decreased accumulated cG250 and decreased number of microvessels in NU12 tumors and very limited necrosis, increased accumulated cG250 and unchanged number of microvessels in SK-RC-52 tumors.

A-D, NU12 control tumors; E-H, NU12 sunitinib treated tumors; I-L, SK-RC-52 control tumors; M-P, SK-RC-52 sunitinib treated tumors. HE staining in A, E, I and M. Sunitinib treatment did not modify CAIX expression in either NU12 or SK-RC-52 (B,F and J,N). In NU12 control tumors (A-D), homogeneous accumulation of cG250 (C) was observed. D: tumor vasculature visualized by staining with 9 F1. In sunitinib treated NU12 tumors (E-H), extensive necrosis was present as judged by HE (E) and accumulated cG250 (G) and microvessels (H) were only observed in the tumor rim. SK-RC-52 control tumors (I-L), revealed focal accumulation of injected cG250 (J) and moderate microvessel density (MVD) as visualized by staining with 9 F1 (L). Accumulation of cG250 was increased in sunitinib treated SKRC52 tumors (O vs. K) and MVD appeared to be increased (P vs. L). Necrosis was limited regardless of treatment (I, M). N: necrosis. Original magnification × 25 and × 200.