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. 2015 Mar 4;17(2):225–237. doi: 10.1016/j.neo.2015.01.001

Figure 5.

Figure 5

Effect of OSM on the expression of Mesenchymal and Proneural signature genes in primary GBM culture. (A) Expression at transcript level of Mesenchymal and Proneural markers in primary culture G1 measured by quantitative real-time PCR. Data is represented as mean +/− SD of two experiments done in duplicates. *P < .05 untreated vs. OSM treated. (B) Protein level variation of Mesenchymal and Proneural markers in primary culture G1 treated with OSM (50 ng/ml) detected by western blot analysis. The blots are representative of three independent experiments. (C) Expression of YKL40 and fibronectin (Mesenchymal markers) in primary culture G1 treated with OSM (50 ng/ml) visualized by immunofluorescence staining. The images are representative of three independent experiments. Magnification 60 ×, Bars 20 μm.