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. 2015 Feb;70(2):136–143. doi: 10.6061/clinics/2015(02)11

Figure 1.

Figure 1

Trypsin-Sepharose 4B affinity chromatography of the tamarind seed trypsin inhibitor (TTI). A) Elution profile of the retained acetone-precipitated fraction (RPA). Adsorbed proteins were monitored at 280 nm (-). The inhibitory activity on trypsin (---) was assayed using 100 µL of TTI. B) Denaturing electrophoresis on a 12% polyacrylamide gel after staining with Coomassie Brilliant Blue R-250. M, Molecular mass markers; PA, precipitated with acetone; RPA, fraction obtained after precipitation with acetone isolated by Trypsin-Sepharose affinity chromatography and TTI, tamarind trypsin inhibitor, isolated by Trypsin-Sepharose affinity chromatography and subjected to dialysis. The arrows indicate 14 kDa for RPA and 20 kDa for TTI.