Figure 4.

Inhibitory effects of scoparone on STAT signaling induced by PDGF in vascular smooth muscle cells (VSMCs). (a, b) Western blot analysis of cell regulatory proteins (a) and different forms of STAT (b) treated with 20 ng ml⁻¹ PDGF±200 μM scoparone for different durations. Total proteins were isolated from VSMCs induced at these differing time points (0–24 h). ppRb indicates phosphorylated retinoblastoma protein (pRb). (c) RT–PCR. Total RNA was isolated from VSMCs incubated with 20 ng ml⁻¹ PDGF±200 μM scoparone for different durations (0–48 h). The mRNA expression levels of MMP-9 and actin as a control were visualized in representative agarose gels.