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. 2015 Mar 27;47(3):e154. doi: 10.1038/emm.2015.7

Figure 2.

Figure 2

Normal thymic development and maturation in T-T TCRmini and T-E TCRmini mice. (a) Thymic developmental profiles of mixed BM chimeras (TCRmini Tg+Tg/Cα−/− → Rag−/−I-Ab−/− for T-T TCRmini; TCRmini Tg+Cα−/− → Rag−/− for T-E TCRmini). These chimeras were killed 6 weeks after BM cell engraftment. Usage of TCRVα2.3 chain was checked in CD4 SP thymocytes of WT, T-E TCRmini and T-T TCRmini mice, and surface expression of CD24, CD44, and CD62L on Vα2.3hi-gated CD4 SP thymocytes was shown as histograms. The data are representative of two independent experiments (numbers of total thymocytes in WT mice: 5.83 × 107 and 6.1 × 107, T-T TCRmini mice: 1.28 × 107 and 6.73 × 107, T-E TCRmini mice: 1.54 × 108 and 1.12 × 108; numbers of CD4 SP thymocytes in T-T TCRmini mice: 8.16 × 104 and 38.3 × 104, T-E TCRmini mice: 24.6 × 104 and 19.1 × 104). (b) Representative developmental profiles of splenic CD4+ and CD8+ T cells from WT, T-E TCRmini, and T-T TCRmini mice. The data are representative of two independent experiments (numbers of total splenocytes in WT mice: 8.0 × 107 and 4.73 × 107, T-T TCRmini mice: 3.6 × 107 and 4.93 × 107, T-E TCRmini mice: 5.75 × 107 and 5.98 × 107; numbers of CD4 T cells in the spleen in T-T TCRmini mice: 19.8 × 104 and 28.2 × 104, T-E TCRmini mice: 30.3 × 104 and 28.9 × 104).