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. 2015 Mar 27;47(3):e154. doi: 10.1038/emm.2015.7

Figure 5.

Figure 5

TCR repertoires of CD4 SP thymocytes derived from T-T or T-E interactions without Vβ fixation. (a) CDR3α length distribution of the CD4 SP thymocytes pooled from two T-T TCRmini mice and two T-E TCRmini mice with a wide range of Vβ elements (referred to as T-T_Op and T-E_Op, respectively) compared with T-T TCRmini and T-E TCRmini mice with fixation (referred to as T-T_Fix and T-E_Fix, respectively) (numbers of total thymocytes in T-T_Op: 6.28 × 107 and 7.03 × 107, T-E_Op: 3.88 × 107 and 7.58 × 107; numbers of CD4 SP thymocytes in the thymus in T-T_Op mice: 8.2 × 104 and 38.3 × 104, T-E_Op mice: 24.6 × 104 and 19.1 × 104). (b) The total number of unique sequences obtained from the CD4 SP thymocytes of the T-T TCRmini and T-E TCRmini mice was assessed based on the number of unique sequences observed (Obs) and the ACE. The empirical P-values were estimated from a one-tailed permutation test.