Table 2. Frequency of parental TCRα chain in Vβ transgenic mouse models.
| Vβ transgenic mouse models: parental TCRα sequence | Reference | ||
|---|---|---|---|
| OT-II TCR-derived TCRβ5.2 transgene: CAARGNRIFF (Vα2.3-Jα31) | Present data | ||
| CD4 SP Thymocytes | Incidence | % of Pop. | |
| T-T TCRmini | 1/376 | 0.27% | |
| T-E TCRmini | 10/467 | 2.14% | |
| TCRmini Tg | 4/137 | 2.92% | |
| OT-I TCR-derived TCRβ5.2 transgene: CAASDNYQL (Vα2.3-Jα26) | 13 | ||
| CD8 SP Thymocytes | Incidence | % of Pop. | |
| Limited mice | 6/100 | 6.00% | |
| MBP-specific TCR-derived TCRβ8.2 transgene: CAASANSG (Vα2.3-Jα11) | 28 | ||
| MBP-TCRβ Transgenic | Incidence | % of Pop. | |
| CD4 SP Thymocytes | 19/29 | 65.52% | |
| CD8 SP Thymocytes | 0/21 | 0.00% | |
| DN32H6 (NK1.1+ T cells)-derived TCRβ8.2 transgene: VVGDRGSA (Vα14-Jα281) | 29 | ||
| CD4+Vβ8+ thymocytes | Incidence | % of Pop. | |
| DN32H6β transgenic #1 | 15/17 | 88.24% | |
| DN32H6β transgenic #2 | 14/18 | 77.78% | |
| Transgene negative littermate | 15/23 | 65.22% | |
Abbreviation: MBP, myelin basic protein; TCR, T cell receptor.