Table III.
Optimization for Fab expression in shake flask culture.
Trial variables | 1 l | 1.25 l | 500 ml | 500 ml |
---|---|---|---|---|
37°C O/N starting culture | 8–9 | 8–9 | 8–9 | / |
30°C O/N starting culture | / | / | / | 6–7 |
24 h sub-culturea | 4–5 | 3–4 | 6–10 | 8–10 |
Protein Yield mg/l | Nil | Nil | 1.2 | 2.5–4 |
aCell Culture readings were taken at OD600 nm.