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. 2011 Jul 23;150(5):535–543. doi: 10.1093/jb/mvr093

Table III.

Optimization for Fab expression in shake flask culture.

Trial variables 1 l 1.25 l 500 ml 500 ml
37°C O/N starting culture 8–9 8–9 8–9 /
30°C O/N starting culture / / / 6–7
24 h sub-culturea 4–5 3–4 6–10 8–10
Protein Yield mg/l Nil Nil 1.2 2.5–4

aCell Culture readings were taken at OD600 nm.