Figure 3.

Cell cycle kinetic is not affected in both Arx cKO VZ and SVZ progenitors, but IPC growth fraction is severely reduced. (A–J″) Cortical fields of BrdU and TBR2 labelings performed on control and Arx cKO cortices at 5 (0.5, 3.5, 6.5, 9.5 and 12.5 h) of the 6 time points of the BrdU cumulative labeling experiment are shown. (K–M) Double labeling for TBR2 and KI67 and quantification of KI67+TBR2+ normalized on total TBR2+ cells reveals a severe reduction of the IPC proliferating fraction in Arx cKO with respect to control. (N and O) Labeling indices (LI) for both control and cKO RG and IPC progenitor (calculated as the ratio between TBR2+ BrdU+ cells on the total of TBR2+ and normalized on the IPC growth fraction in the latter case), are plotted against correspondent BrdU-labeling times and the interpolating lines are drawn. (P) Evaluation of cell cycle duration (Tc) and S-phase duration (Ts) are reported.