Figure 4.

Increased cell cycle exit affects Arx cKO progenitors and the progeny of TBR2+ cells is reduced. (A and B) E14.5 control and Arx cKO cortical sections were double labeled for Ki67 and BrdU, following BrdU injection at E13.5. (C) Quantification of the proportion of cells leaving the cycle (leaving fraction: BrdU+Ki67-/tot BrdU+ cells). (D and E) E15.5 coronal sections and relative higher magnifications (D′ and E′) of control and Arx cKO in the transgenic background Tbr2::GFP analyzed for GFP expression reveal a reduction in intensity and number of GFP+ cells in Arx cKO SVZ, intermediate zone (IZ), and cortical plate (CP) as quantified in F.