Table 1.
Dominant clonally rearranged IGHV and IGLV gene sequences identified in leukemias derived from the various Eμ-TCL1 cohorts
| Mouse | Cohort | HC | IGHV (% hom.) | HCDR3 | LC | IGLV (% hom.) | LCDR3 |
|---|---|---|---|---|---|---|---|
| TCL1-55 | Eμ-TCL1/IgPtC | Transgenic | IGHV12-3 (100%) | CAG-DYDGYWYFDV-W | Endogenous | IGKV4-91 (100%) | CQQ-GSSIPF-TF |
| TCL1-89 | Eμ-TCL1/IgPtC | Transgenic | IGHV12-3 (100%) | CAG-DYDGYWYFDV-W | Endogenous | IGKV4-91 (100%) | CQQ-GSSIPY-TF |
| TCL1-335 | Eμ-TCL1/IgPtC | Transgenic | IGHV12-3 (100%) | CAG-DYDGYWYFDV-W | Endogenous | IGKV4-91 (100%) | CQQ-GSSIPL-TF |
| TCL1-337 | Eμ-TCL1/IgPtC | Transgenic | IGHV12-3 (100%) | CAG-DYDGYWYFDV-W | Endogenous | IGKV4-91 (100%) | CQQ-GSSIPL-TF |
| Endogenous | IGHV1-69 (97.6%) | CAR-WGRWLDY-W | |||||
| TCL1-338 | Eμ-TCL1/IgPtC | Transgenic | IGHV12-3 (100%) | CAG-DYDGYWYFDV-W | Endogenous | IGKV4-91 (100%) | CQQ-GSSIPL-TF |
| TCL1-349 | Eμ-TCL1/IgPtC | Transgenic | IGHV12-3 (100%) | CAG-DYDGYWYFDV-W | Endogenous | IGKV4-91 (100%) | CQQ-GSSIPL-TF |
| TCL1-369 | Eμ-TCL1/IgPtC | Transgenic | IGHV12-3 (100%) | CAG-DYDGYWYFDV-W | Endogenous | IGKV4-91 (100%) | CQQ-GSSIPY-TF |
| TCL1-835 | Eμ-TCL1/IgPtC | Transgenic | IGHV12-3 (100%) | CAG-DYDGYWYFDV-W | Endogenous | IGKV4-91 (100%) | CQQ-GSSIPL-TF |
| TCL1-836 | Eμ-TCL1/IgPtC | Transgenic | IGHV12-3 (100%) | CAG-DYDGYWYFDV-W | Endogenous | IGKV4-91 (100%) | CQQ-GSSIPL-TF |
| TCL1-120 | Eμ-TCL1/IgSm | Endogenous | IGHV5-4 (99.6%) | CAR-DSPLGLYYFDY-W | Endogenous | ND. | ND |
| TCL1-121 | Eμ-TCL1/IgSm | Transgenic | IGHV1-14 (99.3%) | CAR-NGWLPPMDY-W | Endogenous | IGKV14-126 (100%) | CLQ-HGESPY-TF |
| Endogenous | IGHV2-2 (100%) | CAR-ANWDAWFAY-W | |||||
| Endogenous | IGHV11-2 (100%) | CMR-YGYDWYFDV-W | |||||
| TCL1-165 | Eμ-TCL1/IgSm | Transgenic | IGHV1-14 (99.3%) | CAR-NGWLPPMDY-W | Endogenous | IGKV4-57 (100%) | CQQ-RSSYPP-TF |
| Endogenous | IGHV5-9 (100%) | CAR-HYDFDY-W | |||||
| TCL1-166 | Eμ-TCL1/IgSm | Transgenic | IGHV1-14 (99.3%) | CAR-NGWLPPMDY-W | Endogenous | IGKV3-1 (100%) | CQQ-SRKVW-TF |
| TCL1-417 | Eμ-TCL1/IgSm | Transgenic | IGHV1-14 (99.3%) | CAR-NGWLPPMDY-W | Endogenous | IGKV5-43 (100%) | CQQ-SNSWPQL-TF |
| Endogenous | IGHV2-2 (100%) | CAR-SYDGYYGYAMDY-W | |||||
| TCL1-481 | Eμ-TCL1/IgSm | Transgenic | IGHV1-14 (99.3%) | CAR-NGWLPPMDY-W | Endogenous | IGKV5-43 (100%) | CQQ-SNSWPQL-TF |
| Endogenous | IGHV2-2 (100%) | CAR-SYDGYYGYAMDY-W | |||||
| TCL1-685 | Eμ-TCL1/IgSm | Transgenic | IGHV1-14 (99.3%) | CAR-NGWLPPMDY-W | Endogenous | IGKV4-68 (100%) | CQQ-WSSNPMY-TF |
| TCL1-699 | Eμ-TCL1/IgSm | Transgenic | IGHV1-14 (99.3%) | CAR-NGWLPPMDY-W | Endogenous | IGKV4-72 (100%) | CQQ-WSSNPW-TF |
| TCL1-48 | TCL1/IgHEL | Endogenous | IGHV12-3 (100%) | CAG-DRTGYWYFDV-W | Endogenous | IGKV1-135 (100%) | CWQ-GTHFPR-TF |
| Endogenous | IGKV3-7 (99.7%) | CQH-SWEIPL-TF | |||||
| TCL1-217 | TCL1/IgHEL | Endogenous | IGHV6-3 (100%) | CTE-GGNYFDY-W | Transgenic | IGKV5-43 (99.3%) | CQQ-SNSWPY-TF |
| Endogenous | IGHV11-1 (100%) | CAR-YYYYGSSYAMDY-W | |||||
| Endogenous | IGHV1-58 (100%) | CAR-GGGTAXFAY-W | |||||
| TCL1-324 | TCL1/IgHEL | Endogenous | IGHV5-9-1 (100%) | CTR-DWDYFDY-W | Transgenic | IGKV5-43 (99.3%) | CQQ-SNSWPY-TF |
| TCL1-603 | TCL1/IgHEL | Endogenous | IGHV5-9-1 (100%) | CTR-DWDYFDY-W | Transgenic | IGKV5-43 (99.3%) | CQQ-SNSWPY-TF |
| Endogenous | IGHV1S56 (98.3%) | CAR-GGMVYYYAMDY-W | |||||
| Endogenous | IGHV5-17 (100%) | CAS-TARATNYYAMDY-W | |||||
| TCL1-683 | TCL1/IgHEL | Endogenous | IGHV5-9-1 (100%) | CTR-DWDYFDY-W | Transgenic | IGKV5-43 (99.3%) | CQQ-SNSWPY-TF |
| Endogenous | IGHV3-8 (100%) | CAR-YYYGSMDY-W | |||||
| TCL1-941 | TCL1/IgHEL+HEL/CpG | Endogenous | IGHV1S22 (100%) | CTR-LDY-W | Transgenic | IGKV5-43 (99.3%) | CWQ-GTHFPR-TF |
| Endogenous | IGHV1S56 (98.3%) | CAR-GGMVYYYAMDY-W | |||||
| TCL1-356 | TCL1/IgHEL/sHEL | Endogenous | IGHV5-9-1 (100%) | CTR-DWDYFDY-W | Transgenic | IGKV5-43 (99.3%) | CQQ-SNSWPY-TF |
| TCL1-362 | TCL1/IgHEL/sHEL | Endogenous | IGHV5-9-1 (100%) | CTR-DWDYFDY-W | Transgenic | IGKV5-43 (99.3%) | CQQ-SNSWPY-TF |
| TCL1-739 | TCL1/IgHEL/sHEL | Endogenous | IGHV12-3 (100%) | CAG-DSDGYYWFAY-W | Endogenous | IGKV4-91 (100%) | CQQ-GSSIP-TF |
| Transgenic | IGKV5-43 (99.3%) | CQQ-SNSWPY-TF | |||||
| TCL1-947 | TCL1/IgHEL/sHEL | Endogenous | IGHV5-9-1 (100%) | CTR-DWDYFDY-W | Transgenic | IGKV5-43 (99.3%) | CQQ-SNSWPY-TF |
| Endogenous | IGHV1S56 (98.3%) | CAR-GGMVYYYAMDY-W | |||||
| Endogenous | IGHV1S22 (100%) | CTR-LDY-W | |||||
| Endogenous | IGHV5-17 (100%) | CAS-TARATNYYAMDY-W | |||||
| TCL1-749 | TCL1/IgHEL/mHEL-KK | Endogenous | IGHV12-3 (100%) | CAG-DSSSYWYFDV-W | Transgenic | IGKV5-43 (99.3%) | CQQ-SNSWPY-TF |
| Endogenous | IGHV1S81 (100%) | CAR-EDY-W | |||||
| Endogenous | IGHV1S56 (98.3%) | CAR-GGMVYYYAMDY-W |