Table 5.
Stimulation of arachidonic acid release from RAW cells by NAglya (Adapted from Burstein el al., 2008)
NAgly (μM) | Arachidonic Acid (Treated/Control) | SD |
---|---|---|
1.5 | 1.0 | 0.21 |
3.0 | 1.2 | 0.13 |
6.0 | 1.4 | 0.32 |
12.0 | 1.85 | 0.31 |
24.0 | 2.95 | 0.25 |
Following a 2 hr labeling period with 14C-arachidonic acid, the media (RPMI+0.1% BSA) were changed and the cells treated for 1 hr with NAgly in 10μl of DMSO. The control was 10 μl of DMSO. Release was measured by LSC on a 0.1 ml aliquot of medium. Values shown are the means ± SD. The cells were treated with non-radioactive NAgly.