Table 3. Acoustic features of vocalizations from control and Emx1-CRE;Esco2fl/fl (ESCO2) males (mean ± SEM), for the unpartitioned data set (all calls), and separately for the six clusters.
| Acoustic feature | Genotype | All | Cluster1 | Cluster2 | Cluster3 | Cluster4 | Cluster5 | Cluster6 |
|---|---|---|---|---|---|---|---|---|
| Duration [ms] | control | 36.6 ± 0.8 | 29.1 ± 0.8 | 34.6 ± 1.5 | 39.9 ± 1.7 | 31.4 ± 0.1 | 76.1 ± 3 | 30.6 ± 2.1 |
| ESCO2 | 37.9 ± 0.7 | 29.7 ± 0.1 | 39.3 ± 1 | 39.1 ± 1.4 | 30.8 ± 1.0 | 72.3 ± 2.7 | 32.7 ± 1.5 | |
| Ampl. gap [ms] | control | 2.0 ± 0.1 | 0.7 ± 0.1 | 2.1 ± 0.3 | 1.8 ± 0.3 | 0.7 ± 0.1 | 10.6 ± 0.7 | 1.1 ± 0.3 |
| ESCO2 | 1.9 ± 0.1 | 0.5 ± 0.1 | 1.5 ± 0.2 | 1.6 ± 0.2 | 0.4 ± 0.1 | 10.9 ± 0.6 | 0.8 ± 0.1 | |
| PF start [kHz] | control | 73.2 ± 0.4 | 67.8 ± 0.5 | 72.5 ± 1.2 | 65.7 ± 1.4 | 75.0 ±0.3 | 71.7 ± 0.8 | 98.7 ± 1.9 |
| ESCO2 | 74.6 ± 0.5 | 66.8 ± 0.6 | 74.1 ± 0.9 | 64.6 ± 1.3 | 73.9 ± 0.4 | 72.2 ± 1.2 | 98.5 ± 1.2 | |
| PF mean [kHz] | control | 71.8 ± 0.2 | 72.6 ± 0.4 | 75.2 ± 0.9 | 71 ± 1 | 70.1 ± 0.3 | 70.1 ± 0.7 | 77.2 ± 1.2 |
| ESCO2 | 71.8 ± 0.4 | 70.7 ± 0.7 | 7.7 ± 0.1 | 72.3 ± 1.2 | 67.8 ± 0.4 | 70.3 ± 1 | 76.7 ± 1.1 | |
| PF max [kHz] | control | 85.2 ± 0.5 | 79.1 ± 0.5 | 99.0 ± 1.7 | 102.6 ± 2 | 77.6 ± 0.3 | 87.4 ± 1.5 | 102 ± 1.9 |
| ESCO2 | 89.1 ± 0.6 | 76.7 ± 0.8 | 101.4 ± 1.2 | 103.2 ± 1.5 | 75.9 ± 0.4 | 93.5 ± 1.8 | 101.9 ± 1.2 | |
| PF max loc | control | 0.41 ± 0.01 | 0.71 ± 0.01 | 0.66 ± 0.02 | 0.68 ± 0.03 | 0.1 ± 0.01 | 0.48 ± 0.03 | 0.07 ± 0.02 |
| ESCO2 | 0.41 ± 0.01 | 0.72 ± 0.01 | 0.68 ± 0.02 | 0.66 ± 0.03 | 0.09 ± 0.01 | 0.49 ± 0.03 | 0.08 ± 0.01 | |
| PF jump [kHz] | control | 1.3 ± 0.8 | 4.0 ± 0.7 | −3.9 ±2.1 | 43.4 ± 1.6 | −1.6 ± 0.5 | 11.0 ± 2.9 | −2.4 ± 2.7 |
| ESCO2 | −4.2 ± 0.9 | −0.3 ± 0.8 | −3.5 ± 1.4 | 40.7 ± 1.5 | −3.2 ± 0.6 | 7.5 ± 3.2 | −2.6 ± 1.6 | |
| PF modulation [Hz] | control | 372 ± 14 | 220 ± 10 | 845 ± 78 | 898 ± 58 | 197 ± 7 | 337 ± 31 | 555 ± 49 |
| ESCO2 | 461 ± 16 | 192 ± 15 | 847 ± 56 | 857 ± 48 | 199 ± 9 | 431 ± 42 | 544 ± 33 | |
| Slope of trend | control | −0.1 ± 0.01 | 0.2 ± 0.01 | 0.0 ± 0.04 | 0.2 ± 0.05 | −0.3 ± 0.01 | −0.1 ± 0.02 | −0.6 ± 0.09 |
| ESCO2 | −0.1 ± 0.02 | 0.2 ± 0.02 | 0.1 ± 0.03 | 0.3 ± 0.05 | −0.4 ± 0.02 | −0.1 ± 0.02 | −0.6 ± 0.06 | |
| PF sharpness [Hz] | control | 1996 ± 7 | 1978 ± 14 | 2048 ± 22 | 2032 ± 22 | 2004 ± 12 | 1904 ± 17 | 2045 ± 29 |
| ESCO2 | 1956 ± 7 | 1880 ± 13 | 2019 ± 19 | 2008 ± 22 | 1939 ± 14 | 1899 ± 19 | 2009 ± 21 |