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. 2015 Mar 6;5:8850. doi: 10.1038/srep08850

Figure 4. Detection of SRV-4 viral RNA by RT-PCR.

Figure 4

Plasma of five affected macaques (lane: 1–5) were examined by RT-PCR using the primer set, aSRV-F1167 and aSRV-R1710. To confirm the results, same samples were re-tested using another primer set, aSRV-F429, aSRV-R855. Each primer sets amplified a single expected products respectively. In contrast to the affected macaques, two healthy controls reared at the second campus (lane: 6–7) were negative. 1: WK1655 (2008/3/12), 2: TH2158 (2008/3/15), 3: AR1694 (2008/5/15), 4: AR1995 (2008/5/23), 5: TH2041 (2009/6/22). 6: OKN244 (2010/12/14), 7: OKN245 (2010/12/14) 8: H2O. Figures in each parenthesis represent the date of blood collection. Blood from each affected macaques was collected just after onset of thrombocytopenia. Photo courtesy of M.O.