Figure 2.

(A) Immunofluorescence microscopy showing neutrophil extracellular traps, defined as colocalized extracellular DNA (blue), neutrophil elastase (NE) (green), and histone (red), present in the lung after hilar clamp (HC) but not after sham surgery. Scale bar = 10 μm. Results are representative of at least three independent experiments. (B, C) NE-DNA complexes are increased in plasma after HC (C) but are not increased in bronchoalveolar lavage fluid (B). n ≥ 6 for all groups, *P < 0.05. BALF = bronchoalveolar lavage fluid.