Figure 4.

(A) Immunofluorescence microscopy showing neutrophil extracellular traps, defined as colocalized extracellular DNA (blue), neutrophil elastase (NE) (green), and histone (red), present in transplanted lungs subjected to prolonged cold ischemia (PCI), but not in those transplanted immediately after harvest (control group). Scale bar = 10 μm. Results are representative of at least three independent experiments. (B) NE-DNA complexes are increased in bronchoalveolar lavage fluid collected from lung grafts subjected to PCI as compared with lungs transplanted immediately after harvest (n = 6 in each group), whereas NE-DNA complexes are present at similar low levels in plasma from both groups of recipient mice (C) (n ≥ 12). *P < 0.05. BALF = bronchoalveolar lavage fluid.