Figure 5.

(A–C) Photomicrographs (×40 magnification) showing immunohistochemical staining of lung tissue for the platelet-specific marker CD41 in (A) lung transplanted immediately after harvest (control subject), (B) lung subjected to prolonged cold ischemia (PCI) and transplanted into a recipient treated with diluent (dimethyl sulfoxide), and (C) lung subjected to PCI and transplanted into a recipient treated with aspirin (ASA) before transplant. Images are representative of at least three independent experiments. (D) Plasma and (E) bronchoalveolar lavage fluid (BALF) thromboxane B₂ levels are reduced by ASA treatment in mice that received lung transplants after PCI. ASA treatment also reduces (F) BALF neutrophil elastase (NE)-DNA complexes, (G) neutrophils, and (H) protein concentration, and (I) increases recipient arterial partial pressure of oxygen (Po₂). n = 4 for all groups, *P < 0.05, **P < 0.01.