. 2015 Feb 22;13:9. doi: 10.1186/s12958-015-0005-4

Table 1.

Feeder-free culture systems using extracellular matrix (ECM) proteins or biological substrates

System description/substrate	Cell type	Medium/supplements	SF ¹	CD ²	XF ³	Culture period	Ref.
Matrigel-mouse	hESC	MEF –CM⁴ (basal medium DMEM+ KSR⁵) bFGF	Y	N	N	180 days	[6]
Matrigel-mouse	hESC/	DMEM/F12	Y	N	N	20 passages	[102]
Matrigel-mouse	hiPSC	Dorsomorphin, IWP-2, bFGF,, TGF-β1, Activin	Y	N	N	20 passages	[102]
ECM from embryoid body (e-ECM)	hESC	TeSR2	Y	Y	Y	20 passages	[65]
Human fibronectin	hESC	DMEM/KSR	Y	N	N	>47 passages	[69]
Human fibronectin	hESC	bFGF,, TGF-β1 (+/− LIF)	Y	N	N	>180 days	[69]
Laminin	hESC	DMEM/KSR	Y	N	N	>20 passages	[51]
Laminin	hESC	bFGF, Activin	Y	N	N	>20 passages	[51]
Laminin,vitronectin, fibronctin and collagen IV (defined human ECM)	hESc	TeSR1	Y	N	Y	11–25 passages	[70]
Human placental ECM	hESC	XF medium -Human plasma extract, with DMEM/F12 base, bFGF, TGFβ	N	N	Y	39 passages	[68]
Laminin isoforms 111,332,511	hESC/	MEF –CM (basal medium DMEM+ KSR⁵)	N	N	N	10 passages	[72]
	hiPSC	MEF –CM (basal medium DMEM+ KSR⁵)
	hiPSC	bFGF
Laminin E8 fragments (LM-E8)	hESC/	mTeSR1,	Y	N	N		[74]
	hiPSC	StemPro,	Y	N	N	10–30 passages
	hiPSC	TeSR2	Y	Y	Y
E-cadherin	hESC	mTeSR1	Y	N	N	>90 days	[79]
E-cadherin	hiPSC		Y	N	N	>90 days	[79]
Laminin-511	hESC	TeSR1	Y	N	Y	20 passages	[71]
Laminin-521/E-cadherin matrix	hESC	mTeSR1,	Y	N	N	35 passages	[80]
Laminin-521/E-cadherin matrix	hiPSC	TeSR2,	Y	Y	Y	12–15 passages	[80]
Vitronectin	hESC	mTeSR1	Y	N	N	10 passages	[82]
Vitronectin- truncated	hESC	E8 medium	Y	Y	Y	>25 passages	[83]
Vitronectin- truncated	hiPSC	E8 medium	Y	Y	Y	>25 passages	[83]
Fibronectin,, laminin and vitronectin	hESC	DMEM/F12 +/− bFGF	Y	Y	Y	>24 passages	[87]
Fibronectin,, laminin and vitronectin	hESC	Wnt/ID8	Y	Y	Y	>24 passages	[87]
Recombinant human vitronectin	hESC/	E8 medium	Y	Y	Y	>30 passages	[85]
Alginate microencapsulation	hESC	MEF-CM (basal medium DMEM//KSR)	Y	N	N	14 days	[126]
Alginate encapsulation of hESC on		bFGF	Y	N	N	19 days
Matrigel coated microcarriers		bFGF	Y	N	N	19 days
Aliginate-chitosan scaffold-3D	hESC	DMEM/F12 + KSR + FBS + bFGF	Y	N	N	21 days	[114]
Alginate-chitosan microfiber scaffold-3D	hiPSC	mTeSR1	Y	N	N	10 passages	[115]
Alginate-chitosan microfiber scaffold-3D	hiPSC	ROCK inhibitor	Y	N	N	10 passages	[115]
Alginate encapsulated	hESC	DMEM + KSR + bFGF	Y	N	N	>260 days	[116]
Nanofibrillar cellulose	hESC	mTeSR1	Y	N	N	>26 days	[117]
Aggregates/ in suspension Fibronectin and laminin present	hESC	Neural basal medium.Neutrodoma-CS	Y	N	Y	>10 weeks	[128]
Aggregates/ in suspension Fibronectin and laminin present	hESC	bFGF, Activin, Neurotrophins BDNF, NT3, NT4	Y	N	Y	>10 weeks	[128]
Aggregates in suspension	hESC	mTESR1	Y	N	N	20–50 passages	[131]
Polymer to prevent fusion/	hiPSC/	StemPro	Y	N	N		[131]
DE-53 Matrigel coated cellulose microcarrier	hESC	mTeSR1	Y	N	N	25 passages	[125]
DE-53 Matrigel coated cellulose microcarrier	hESC	StemPro	Y	N	N		[125]
Artificial spider silk + vitronectin	hESC/	Nutirstem XF/FF	Y	N	Y	>30 passages	[93]
Artificial spider silk + vitronectin	hiPSC	Nutirstem XF/FF	Y	N	Y	>30 passages	[93]

¹SF serum –free medium.

²CD chemically defined medium (excludes any media containing BSA or HSA fraction due to their unknown and variable composition).

³XF xeno-free, no animal components in medium or culture system.

⁴MEF-CM mouse embryonic fibroblast conditioned medium.

⁵KSR knock out serum replacement protein.