Table 2.

Synthetic substrates for hESC culture

System description/substrate	Cell type	Medium/supplements	SF 1	CD 2	XF 3	Culture period	Ref
Synthetic acrylate surfaces (PAS) with peptides: Vitronectin, bone sialo protein	hESC	X -VIVO 10	Y	N	Y	>10 passages	[89]
		bFGF,TGF-β1
		Activin A
Synthemax 4	hiPSC	mTeSR1	Y	N	N	10 passages	[90]
Synthemax 4	hESC	mTESR1	Y	N	N	10–20 passages	[91]
	hiPSC	TESR2	Y	Y	Y
		PSGro	Y	Y	Y
		Nutristem XF	Y	N	Y
PMEDSAH 5 coated plates	hESC	MEF-CM6	N	N	N	25 passages	[98]
		hCCM7	N	N	Y
		StemPro,	Y	N	N
		mTeSR	Y	N	N
Alkanethiol with heparin binding proteins	hESC	mTeSR1	Y	N	N	>17 passages	[92]
		Rock inhibitor
PMVE-alt-MA 8	hiPSC	StemPro	Y	N	N	5 passages	[94]
APMAAm 9 hydrogels	hESC	mTeSR1	Y	N	N	20 passages	[95]
Polyethylene glycol hydrogels (PEG)	hESC	DMEM/F12 + KSR	N	N	N	9 days	[118]
		bFGF
Hillex 10 microcarrier//suspension culture	hESC	KSR-XF 10	Y	N	Y	6–14 days	[123]
		BRASTEM,	Y	N	Y
		bFGF

¹SF serum –free medium.

²CD chemically defined medium (excludes any media containing BSA or HSA fraction due to their unknown and variable composition).

³XF xeno-free, no animal components in medium or culture system.

⁴Synthemax acrylate surface coated with peptide derived from vitronectin.

⁵PMEDSAH Poly [2-(methacryloyloxy) ethyl dimethyl-(3-sulfopropyl) ammonium hydroxide]—.

⁶MEF-CM mouse embryonic fibroblast conditioned medium.

⁷Human cell conditioned medium.

⁸PMVE-alt-MA poly (methyl vinyl ether-alt-maleic anhydride.

⁹APMAAm aminopropylmethacrylamide—.

¹⁰Hillex polystyrene beads modified with cationic trimethyl ammonium.