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. Author manuscript; available in PMC: 2016 Feb 2.
Published in final edited form as: Mol Pharm. 2015 Jan 20;12(2):403–410. doi: 10.1021/mp500766x

Figure 5.

Figure 5

Ex vivo examination of FSHR expression. (A) Immunofluorescence FSHR/CD31 double-staining of the CAOV-3, SKOV-3, MDA-MB-231, and PC-3 tumor sections. FSHR-mAb and FITC-labeled goat anti-mouse IgG were used for FSHR staining (green). Subsequently, the tissue slices were stained with rat anti-mouse CD31 antibody and Cy3-labeled donkey anti-rat IgG (red). All images were acquired under the same conditions and are displayed on the same scale. Scale bar: 50 μm. The FSHR fluorescence-tumor uptake correlation curve was also given here with the quantification of fluorescence by ImageJ. (B) RT-PCR assessment of FSHR expression in different tumors. FSHR expression in different tumors was visualized in 1% agarose gel by ethidium bromide. Correlation of relative tumor tissue FSHR expression (via FSHR/GAPDH ratio) and the average %ID/g values of tumor 64Cu-NOTA-FSHR-mAb uptake (measured by PET) at 48 h p.i. was also shown.