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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: J Neurochem. 2015 Jan 27;132(4):429–442. doi: 10.1111/jnc.13006

Fig. 3.

Fig. 3

P7 ethanol treatment reduces the protein levels and differentially regulates mRNA levels of DNMT1 and DNMT3A in mouse brain regions. (a and b) Western blot analysis of DNMT1 and DNMT3A in hippocampus (HP) and neocortex (NC) nuclear extracts obtained 4–24 h after the first saline or ethanol injection. Ponceau S staining confirmed equal loading and β-actin were used as loading controls. (c and d) qPCR analysis of DNMT1 and DNMT3A mRNA in HP and NC extracts obtained 4–24 h after the first saline or ethanol injection groups (n = 10 pups/group). For 0 h ethanol group, saline was injected. [*p < 0.05 vs. saline (0 h) group]. Error bars, SEM. (One-way ANOVA with Bonferroni’s post hoc test).