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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: J Neurochem. 2015 Jan 27;132(4):429–442. doi: 10.1111/jnc.13006

Fig. 4.

Fig. 4

Pharmacological inhibition of caspase-3 rescues P7 ethanol-induced degradation of DNMT1 and DNMT3A proteins as well as DNA methylation in the neonatal mouse brain. (a and b) P7 Mice pre-treated for 30 min with Q-VD-OPh (1 mg/kg) or vehicle were exposed to ethanol and DNMT1 and DNMT3A proteins levels were determined in nuclear extracts of hippocampus (HP) and neocortex (NC) by a Western blot analysis. β-actin was used as a loading control. (c) Global DNA methylation quantification was performed in HP and NC DNA from saline (S), ethanol (E), S + Q-VD-OPh, or E + Q-VD-OPh groups (n= 10 pups/group) (*p < 0.05 vs. S; #p < 0.05 vs. E. Error bars, SEM. (Two-way ANOVA with Bonferroni’s post hoc test).