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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: J Neurochem. 2015 Jan 27;132(4):429–442. doi: 10.1111/jnc.13006

Fig. 5.

Fig. 5

Pharmacological inhibition of G9a rescues P7 ethanol-induced degradation of DNMT1 and DNMT3A proteins and DNA hypomethylation in the neonatal mouse brain. (a and b) P7 Mice pre-treated for 30 min with Bix (1 mg/kg) or vehicle (V) were exposed to saline (S) ethanol (E) and DNMT1 and DNMT3A protein levels in nuclear extracts were determined by a Western blot analysis in hippocampus and neocortex (n= 6 pups/group). β-actin was used as a loading control. (c) Global DNA methylation quantification was performed in hippocampus and neocortex DNA samples from S + V, E + V, S + Bix or E + Bix groups (n= 10 pups/group) (*p < 0.05 vs. S + V; #p < 0.05 vs. E + V). Error bars, SEM. (Two-way ANOVA with Bonferroni’s post hoc test). HP, hippocampus; NC, neocortex.