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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: J Neurochem. 2015 Jan 27;132(4):429–442. doi: 10.1111/jnc.13006

Fig. 6.

Fig. 6

Blocking CB1 receptor with SR rescues ethanol-induced degradation of DNMT1 and DNMT3A proteins and impaired DNA methylation in the neonatal mouse brain. (a and b) Western blot analysis of DNMT1 and DNMT3A proteins and β-actin (loading control) in hippocampal and neocortical nuclear extracts from the four (S+V, E+V, S+SR and E+SR) groups (n = 15 pups/group). P7 mice were treated with ethanol for 8 h, and SR was pretreated for 30 min before the ethanol treatment. (c) Global DNA methylation quantification was performed in hippocampal and neocortical DNA from saline (S), ethanol (E), S + SR, or E + SR groups (n= 10 pups/group) (*p < 0.05 vs. S + V; #p < 0.05 vs. E + V. Error bars, SEM. (Two-way ANOVA with Bonferroni’s post hoc test). HP, hippocampus; NC, neocortex.