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. Author manuscript; available in PMC: 2015 Nov 10.
Published in final edited form as: Angew Chem Int Ed Engl. 2014 Jul 17;53(46):12451–12455. doi: 10.1002/anie.201404349

Figure 3.

Figure 3

PtV-Chip for detection of CYFRA21-1. a) A standard sandwich ELISA was employed in PtV-Chip for biomarker detection. CYFRA21-1 (blue) was added in the assay wells in the bottom plate and bound to the capture antibodies (purple). The PtNP-antibody complexes were loaded into the assay chamber to form sandwich structures. To detect the target biomarkers, PtNP was conjugated with the probe antibody to react with the hydrogen peroxide and produce oxygen gas. b) Image of PtV-Chip after loading reagents and samples. Scale bar, 1 cm. c) Images for single-plex V-Chip results in the absence or presence of different concentration of CYFRA21-1 (0.5, 1, 3, 10, 25, 50 ng·mL−1). d) The CYFRA21-1 calibration curve corresponding to ink advancement at 10 min, with the CYFRA21-1 concentration varying from 0.5 to 50 ng·mL−1. e) Detection results of spiked CYFRA21-1 in PBS buffer and serum. The error bars in d and e represent the SD of three measurements.