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. 2015 Mar 4;6:6228. doi: 10.1038/ncomms7228

Figure 6. PC species containing VLC-PUFAs are reduced in retinas/RPE cups of AdipoR1−/− mice.

Figure 6

Photoreceptor PCs have DHA esterified at the sn-2 position and VLC-PUFAs at sn-1. LC-MS/MS analysis of 24-day-old mouse retina/REP cups shows differences in PC molecular species containing 52–60 carbons. Note that FAs with up to 38 carbons occur at the sn-1 position and 22:6 (22 carbons with six double bonds) is always esterified at the sn-2 position of these retinal PCs. Therefore, only the VLC-PUFAs esterified at sn-1 of these PCs are denoted along the horizontal axis (see inset at lower right in a for retinal PC structure). Notice the decline of PCs in the AdipoR1−/− retinas and RPE cups (a,c). Unusual VLC-PUFA-containing PCs are presented separately. Note that these species increase in the −/− retinas and RPE cups (b,d). Some molecules are highlighted in red to correspond to the molecular structures of Fig. 5. The molecules denoted in blue correspond to the ‘odd’ molecular structures of Supplementary Fig. 3. (n=5, error bars represent s.e.m., *P<0.05 and **P<0.001, determined by t-test.)