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. 2015 Mar 5;6:4. doi: 10.1186/s13100-015-0035-7

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© Schmitt et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Gel electrophoretic separation of rec- and np9 -specific PCR products amplified from a panel of cDNAs. Generated from 15 normal human tissues and peripheral blood leukocytes. Expected product sizes were ca. 580 and 360 bp for rec and np9, respectively. cDNA generated from GCT-derived Tera-1 cell line, known to strongly express HML-2 both on the RNA and protein level (for instance, see ref. [28]), served as a positive control. ‘ctrl.’ indicates a PCR control reaction without template DNA. A faint band representing rec-specific PCR product from PBL is not properly reproduced. ‘n.a.’ indicates additional PCR products of approx. 1 kb in the liver and testis and approx. 2.2 kb in the spleen and the thymus not further investigated in this study.