Table 1.
Positive and negative sera for Triatoma virus by country of origin resulting from different types of analysis
| Country | Sera analysed | Chagas patients (%) | ELISA 1 positive | ELISA 2 negative | WB positive | RT-PCR negative | Negative control |
|---|---|---|---|---|---|---|---|
| Argentina | 122 | 40 | 41 | 50 | 36 | 50 | 12 |
| Bolivia | 117 | 68 | 11 | - | - | - | 20 |
| Brazil | 50 | 100 | 0 | - | - | - | 22 |
| Cuba | 10 | 0* | 0 | - | - | - | 5 |
| Mexico | 100 | 70 | 9 | - | - | - | 8 |
| Portugal | 11 | 100# | 0 | - | - | - | 8 |
| Total | 410 | 63 | 61 | 50 | 36 | 50 | 81 |
Column 1 identifies the country of origin for the samples; results of the TrV sera analyses. Numbers in columns 4–7 indicate the number of serum samples employed in each analysis. ELISA assay 1 corresponds to reactivity against structural proteins, and ELISA assay 2 corresponds to reactivity against the non-structural protein RdRp. WB refers to immunoblotting analysis against the structural proteins of TrV. RT-PCR refers to reverse transcriptase-PCR*. Cuba is a non-endemic country for Chagas disease. #The sera from Portugal are imported case of Chagas disease, mainly from Brazil.