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Agarose gel electrophoresis separation of GAGs (glycosaminoglycan). Glycosaminoglycans were extracted and purified from aortas as described in experimental procedures. Purified GAGs were dissolved in distilled water at a concentration of 1 mg/ml, and 10 μl was layered on agarose gel. A representative electrophoresis shows from the top chondroitin sulphate (CS), dermatan sulphate (DS), heparin sulphate (HS) standard; control (C) and hyphercholesterolaemic group (H-Chol).
