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. 2014 Mar 7;95(3):181–190. doi: 10.1111/iep.12072

Figure 5.

Figure 5

(a) Analysis of TNF-α mRNA expression by semiquantitative RT-PCR. Aortic tissue was extracted as described in experimental procedures, and RT-PCR was performed with TNF-α and β-actin specific primer pairs. Each column of densitometric analysis represents the relative amount of TNF-α in control – □ – aortic tissue and in the H-Chol – ■ – group, normalized to β-actin. Results are expressed as means ± SD for duplicate samples from three independent experiments. #P < 0.05. (b) Analysis of TNF-α protein expression in isolated aortic cells by Western blot. Each column of densitometric analysis represents the relative amount of TNF-α in control – □ – aortic cells and in the H-Chol – ■ – group, normalized to β-actin protein content. Results are expressed as means ± SD for duplicate samples from three independent experiments. *P < 0.05.