Table 1.
Summary of the various histological and biochemical parameters in different groups of animals
| Parameter | Group 1 | Group 2 | Group 3 | Group 4 | P-values | |
|---|---|---|---|---|---|---|
| Histopathology | ||||||
| Pancreas | N | 14 | 12 | 14 | 16 | (1 vs. 2, 1 vs. 3, 1 vs. 4, 2 vs. 3, 2 vs. 4, 3 vs. 4 – P < 0.001) |
| Mean (SD) | 0.33 (0.05) | 7.83 (1.01) | 9.38 (0.78) | 11.75 (1.69) | ||
| Lung | N | 14 | 12 | 14 | 16 | (1 vs. 2, 1 vs. 3, 1 vs. 4, 2 vs. 3, 3 vs. 4 – P < 0.01) (2 vs. 4 – P = 0.136) |
| Mean (SD) | 0.10 (0.05) | 1.89 (0.26) | 1.04 (0.17) | 2.39 (0.49) | ||
| Kidney | N | 14 | 12 | 14 | 16 | (1 vs. 2, 1 vs. 3, 1 vs. 4, 2 vs. 3, 2 vs. 4 – P < 0.01) (3 vs. 4 – P = 0.401) |
| Mean (SD) | 0 (0) | 1.07 (0.29) | 1.08 (0.19) | 2.01 (0.50) | ||
| Apoptosis (TUNEL) | N | 6 | 12 | 10 | 13 | (1 vs. 2 – P = 0.002) (1 vs. 3 – P = 0.005) (2 vs. 4 – P = 0.031) |
| Mean (SD) | 38.51 (6.58) | 50.40 (4.92) | 51.89 (5.06) | 43.39 (5.55) | ||
| Percentage of necrosis | N | 9 | 10 | 12 | 13 | (1 vs. 2, 1 vs. 3, 1 vs. 4 – P < 0.001) (2 vs. 4 – P = 0.006) (3 vs. 4 – P = 0.019) (2 vs. 3 – P = 1) |
| Mean (SD) | 0 (0) | 19.97 (1.60) | 20.36 (1.56) | 37.49 (4.68) | ||
| MPO (U/mg of protein) | ||||||
| Pancreas | N | 12 | 12 | 14 | 16 | (1 vs. 2, 1 vs. 4, 2 vs. 4, 3 vs. 4 – P < 0.001) (1 vs. 3 – P = 0.003) (2 vs. 3 – P = 0.039) |
| Mean (SD) | 0.46 (0.14) | 5.10 (1.06) | 2.38 (0.45) | 12.73 (2.36) | ||
| Lung | N | 8 | 12 | 14 | 16 | (1 vs. 2 – P = 0.001) (1 vs. 3, 1 vs. 4, 2 vs. 3, 3 vs. 4 – P < 0.001) |
| Mean (SD) | 1.79 (0.60) | 6.24 (1.23) | 15.66 (2.24) | 31.23 (4.01) | ||
| Plasma Amylase (U/ml) | N | 9 | 11 | 14 | 12 | (1 vs. 2, 1 vs. 3, 1 vs. 4 – P < 0.001) (2 vs. 3, 2 vs. 4 – P = 0.001) (3 vs. 4 – P = 1) |
| Mean (SD) | 1059.8 (198.33) | 2257.80 (465.82) | 6299.79 (1167.94) | 6714.00 (757.81) | ||
Group 1 represents the control animals, Group 2 represents the animals that received four injections and were sacrificed at the 5th hour, animals of Group 3 received four injections but were sacrificed at the 9th hour and animals of Group 4 received eight injections and were sacrificed at the 9th hour. N, Number of animals; SD, standard deviation; TUNEL, terminal deoxyuridine nick end labelling; MPO, myeloperoxidase; U, units; vs., versus.