Skip to main content
. Author manuscript; available in PMC: 2015 Mar 6.
Published in final edited form as: Biotechniques. 2014 Feb 1;56(2):61–passim. doi: 10.2144/000114133

Figure 2. DNA library preparation using a transposase-based method (Nextera) developed by Illumina.

Figure 2

The transpososome complex comprises an engineered transposase pre-loaded with two double-stranded sequencing adapters. The transpososome simultaneously fragments the DNA and inserts the adapters. The full Illumina adapter sequences are completed during subsequent PCR cycling, after which the library is ready for quantitation and loading onto the flow cell.