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. 2015 Mar 6;10(3):e0119000. doi: 10.1371/journal.pone.0119000

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© 2015 Sharma et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — Schematic representation of genomic oriC region, IRR plasmids and predicted organisation of the oriC region following integration of different IRR plasmids. Southern blot analysis of EcoRI-PstI digested DNA probed with DIG-labelled probes targeting ampR (panel A) and oriC region (panel B). Smaller oriC plasmids were stable in M. bovis till p15-p20, but some integration appeared to have occurred in the oriC region. Lane 1, untransformed M. bovis; lanes, 2, 6 and 8, pIRR5632, pIRRPG2 and pIRR45, respectively. Lanes 3–5: pIRR5632 M. bovis transformants at p10, p15 and p20; lane 7, pIRRPG2 M. bovis transformants at p15; lanes 9–11, pIRR45 M. bovis transformants at p10, p15 and p20.