Heat map for the top 500 most variably methylated loci in penile cancers shows three pathologically defined clusters, a hypermethylated lymph node negative (right (upper green bar)), lymph node positive hypomethylated group (centre (upper gold bar)) and a HPV associated cluster (left (lower two bars (red = 1st-HPV positive, blue = HPV negative, black = 2nd HPV Viral Load, High HPV (>1 copy/cell), white = low (<1copy/cell) grey, no HPV detectable). B) Heatmap of methylation values for 962 significant MVP between node negative (green upper bar), and node positive (gold upper bar).