Table 4.
Multiple reaction monitoring (MRM) transitions for quantification of the nine target compounds
| Compound abbreviations* | Monitoring Mode | Parent (m/z) | Product (m/z) | Tube lens (V) | Collision energy (eV) | Retention times (min) |
|---|---|---|---|---|---|---|
| CCS | [M + H]+ | 285.16 | 270.30 | 99 | 23 | 6.43 |
| CCSG | [M + H]+ | 447.24 | 285.30 | 98 | 20 | 4.89 |
| FAN | [M + H]+ | 623.44 | 381.40 | 130 | 40 | 4.89 |
| PAL | [M + H]+ | 352.23 | 336.30 | 89 | 29 | 5.44 |
| 5-O-M | [M + H]+ | 453.27 | 291.30 | 108 | 22 | 6.01 |
| ONO | [M + H]+ | 431.25 | 269.30 | 97 | 17 | 6.00 |
| FOR | [M + H]+ | 269.20 | 197.30 | 97 | 34 | 7.32 |
| AST-IV | [M + Na]+ | 807.53 | 627.40 | 221 | 49 | 8.69 |
| BER | [M + H]+ | 336.19 | 320.28 | 94 | 30 | 5.38 |
*The compound abbreviations and their associated terms:
AST-IV: astragaloside IV; BER: berberine; CCS: calycosin; CCSG: calycosin-7-O-β-D- glucoside; FAN: fangchinoline; FOR: formononetin; ONO: ononin; PAL: palmatine; 5-O-M: 5-O-methylvisammioside.