Effect of cryopreservation on immunophenotype of TSGs and bone metastasis. TSGs from case 1 at passage 6 were cryopreserved for several weeks and then implanted into mice. a H&E-staining of parent tumor of case 1 RCC. b, c Human-specific Ku70 and CAIX immunohistological stainings of a, respectively. d H&E-stained passage 6 TSG after cryopreservation showed similar phenotype to parent tumor. e, f Ku70 and CAIX immunohistological stainings of d, respectively. g H&E-stained TSG that metastasized to bone after cryopreservation. h, i Ku70 and CAIX immunohistological stainings of g, respectively. j H&E-stained TSG that metastasized to lung after cryopreservation. k, l Ku70 and CAIX immunohistological stainings of j, respectively. Magnification is ×20