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. 2015 Mar 5;13:80. doi: 10.1186/s12967-015-0437-9

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© Bissa et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Functional virus-specific T-cell responses to E6. IFN-γ production was measured in an ELISPOT assay after preventive immunization to detect CD8⁺ T-cells after specific antigenic stimulation with the E6 protein and peptides. The numbers of IFN-γ-secreting spot-forming cells (SFCs) were determined after subtracting the values obtained using the scFv unrelated protein. Data are presented as fold-increased responses to the E6-specific protein and peptides. G2 and G3 are the protocols for the DNA/FP and FP/FP prime/boost vaccinations, respectively. Statistical significances using the ANOVA parametric test are shown: **p <0.01; ***p <0.001.