Fig 2. 2-ME causes a dose- and endothelium-dependent relaxation.
A. Aorta was preconstricted with phenylephrine (Phe, 100 nM) and wall tension was recorded. B. Representative changes in wall tensions of rat aorta induced by a cumulative application of 2-ME in concentrations from 10−7 to 10−5 M. All arteries were preconstricted with Phe (100 nM) before testing with 2-ME. C. Endothelial cell layer was disrupted with a stainless steel wire. Then arteries were preconstricted with Phe (100 nM) before testing with 2-ME (10−5 M). All the experiments were repeated six times with consistent results.