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. 2015 Mar 6;11(3):e1004742. doi: 10.1371/journal.ppat.1004742

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© 2015 Diaz et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 3 — Localization of HA-tagged ESCRT proteins expressed from their endogenous promoters in vps4Δ yeast transformed with (A) empty plasmids or (B) 1a. Yeast were immunostained for the HA-tagged ESCRT proteins (green) and BMV 1a (red) while DNA was stained with DAPI (blue). Images were cropped just outside the yeast cell wall to include only one cell. Scale bars: 2 μm. (C) Localization of HA-tagged ESCRT proteins in snf7 Δ yeast transformed with BMV 1a and Vps4^E233Q-FLAG. Scale bars: 2 μm. (D) Localization of HA-tagged Snf7^L231/L234 proteins in snf7 Δ yeast transformed with BMV 1a and Vps4^E233Q-FLAG. Scale bars: 2 μm.