Fig 4. Snf7p immunoprecipitates with BMV 1a but not with Rtn1p.
(A) vps4Δ cells expressing HA-tagged ESCRTs and either empty plasmids or 1a were lysed and the cleared lysates were subjected to immunoprecipitation using anti-1a or anti-HA antibodies (IP 1a and IP HA, respectively). The resulting immunoprecipitates were analyzed on 4–15% SDS-PAGE and immunoblotted using anti-1a or anti-HA antibodies. (B) Yeast expressing Rtn1p-GFP were transformed with plasmids encoding Snf7p-HA and either an empty plasmid or 1a. Cells were lysed, IP was performed using an anti-GFP antibody, and the resulting IPs were immunoblotted using anti-1a, anti-GFP or anti-HA antibodies. The amount of total lysate used for blotting in the lower control lanes was 1/20th of the lysate volume used for the IPs.