Table 1. Proportion of bacterial taxa with positive virtual amplification in the wgs-embl-pro database revealed by ecoPCR.
Taxonomic rank | No. of taxa | Primer set 1406F/23Sr | Primer set ITSF/ITSReub | Primer set S-D-Bact-1522-b-S-20/L-D-Bact-132-a-A-18 | |||
---|---|---|---|---|---|---|---|
0 mismatch | 1 mismatch | 0 mismatch | 1 mismatch | 0 mismatch | 1 mismatch | ||
phylum | 19 | 84.2 | 89.5 | 36.8 | 68.4 | 0 | 52.6 |
class | 32 | 78.1 | 87.5 | 43.8 | 59.4 | 0 | 46.9 |
order | 72 | 83.3 | 91.7 | 44.4 | 63.9 | 0 | 31.9 |
family | 163 | 79.8 | 87.1 | 49.7 | 64.4 | 0 | 34.4 |
genus | 483 | 65.4 | 72.7 | 33.3 | 48.9 | 0 | 16.8 |
species | 1389 | 48.2 | 56.7 | 28.9 | 40.3 | 0 | 12.0 |