Fig 5. Differentiation potential of hADSCs cultured on a chitosan-based matrix.

a) Alcian blue staining of acid mucopolysaccharide aggregates present in the differentiatedchondrocyte cultures grown on chitosan hydrogel. b) Calcium deposits in the ECM were stained using Von kossa staining of the differentiated osteocytes on the hydrogel matrix.c) Lipid droplets were formed on cells laden on matrix after adipogenic induction and werestained with oil red O staining. Negative staining of un-induced cultures for the respectivelineages is also shown. Magnification, 400X (n = 5).