Table 1. Homology of differentially expressed cDNA-AFLP fragments with known gene sequences in database using BLASTN algorithm along their expression patterns in roots from Citrus grandis seedlings treated with two B × Al levels.
| TDF# | Genebank ID | E value | Max score | Organism origin | Size (bp) | Description | Identity | Fold change | |||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 2.5 B -Al | 2.5 B + Al | 20 B - Al | 20 B + Al | ||||||||
| Metabolism | |||||||||||
| 157-6 | XP_006479398 | 2.E-30 | 118 | Citrus sinensis | 185 | Flavonol synthase/flavanone 3-hydroxylase-like | 100% | 0 b | 1.00 a | 0 b | 1.03 a |
| 134-14 | NP_197540 | 5.E-15 | 76.3 | Arabidopsis thaliana | 171 | Flavanone 3 hydroxylase-like protein | 65% | 1.00 b | 2.80 a | 0.07 c | 0.21 c |
| 149-2 | XP_006487080 | 7.E-29 | 115 | Citrus sinensis | 255 | Probable carboxylesterase 12-like | 81% | 1.00 b | 1.08 ab | 0.12 c | 1.22 a |
| 216-2 | XP_006490283 | 3.E-41 | 147 | Citrus sinensis | 236 | Carboxylesterase 1-like | 95% | 1.00 b | 0.16 c | 0.16 c | 1.47 a |
| 250-3 | XP_006468458 | 4.E-51 | 179 | Citrus sinensis | 297 | Lecithin-cholesterol acyltransferase-like 4-like | 99% | 1.00 a | 0.45 b | 1.10 a | 1.01 a |
| 51-12 | YP_740484 | 9.E-24 | 101 | Citrus sinensis | 162 | Acetyl-CoA carboxylase carboxyltransferase beta subunit | 98% | 1.00 a | 0.05 b | 0.05 b | 0.05 b |
| 136-3 | XP_006492541 | 2.E-39 | 145 | Citrus sinensis | 239 | Adenosylhomocysteinase-like | 91% | 1.00 b | 1.06 b | 0.10 c | 5.97 a |
| 141-5 | XP_006471128 | 6.E-50 | 172 | Citrus sinensis | 279 | Probable S-adenosylmethionine-dependent methyltransferase At5g37990-like | 95% | 1.00 a | 0.08 b | 0.09 b | 0.08 b |
| 87-2 | NP_180524 | 8.E-33 | 129 | Arabidopsis thaliana | 279 | Phosphomethylpyrimidine synthase | 76% | 1.00 a | 0.10 b | 0.11 b | 0.10 b |
| 138-5 | XP_006469907 | 5.E-20 | 90.9 | Citrus sinensis | 256 | Nicotianamine aminotransferase A-like isoform X3 | 75% | 0 b | 1.00 a | 0 b | 1.02 a |
| 138-3 | NP_567934 | 4.E-44 | 158 | Arabidopsis thaliana | 276 | LL-diaminopimelate aminotransferase | 83% | 1.00 a | 1.05 a | 1.06 a | 0.13 b |
| 134-12 | XP_007043658 | 2.E-24 | 103 | Theobroma cacao | 256 | Tyrosine transaminase family protein | 80% | 1.00 a | 1.08 a | 1.10 a | 0.06 b |
| 18-2 | XP_006466965 | 1.E-41 | 143 | Citrus sinensis | 225 | Thiosulfate sulfurtransferase 18-like isoform X1 | 92% | 1.00 a | 0.16 b | 0.17 b | 1.07 a |
| 178-4 | XP_006466965 | 9.E-41 | 141 | Citrus sinensis | 225 | Thiosulfate sulfurtransferase 18-like isoform X1 | 92% | 1.00 a | 0.22 b | 0.18 b | 1.03 a |
| 54-2 | XP_002308954 | 2.E-24 | 101 | Populus trichocarpa | 189 | 40S ribosomal protein S2 | 84% | 1.00 c | 15.58 a | 4.60 b | 15.11 a |
| 80-2 | XP_003523292 | 2.E-56 | 184 | Glycine max | 313 | 60S ribosomal protein L10 | 95% | 1.00 b | 1.03 b | 6.49 a | 1.09 b |
| 201-1 | NP_564355 | 1.E-30 | 119 | Arabidopsis thaliana | 201 | Ferredoxin-NADP reductase, root isozyme 2 | 90% | 1.00 a | 1.03 a | 0.16 b | 1.09 a |
| 25-4 | ACG28186 | 3.E-32 | 121 | Zea mays | 212 | Cytochrome b6-f complex iron-sulfur subunit | 85% | 1.00 a | 0.18 b | 0.08 b | 0.09 b |
| 29-2 | ACG28186 | 5.E-31 | 118 | Zea mays | 212 | Cytochrome b6-f complex iron-sulfur subunit | 84% | 1.00 a | 0.27 b | 0.24 b | 0.21 b |
| 134-9 | XP_002518810 | 3.E-18 | 87.8 | Ricinus communis | 232 | Electron transporter, putative | 75% | 1.00 b | 1.02 b | 1.13 b | 21.82 a |
| 51-9 | XP_002531030 | 2.E-16 | 76.3 | Ricinus communis | 175 | Ribulose-bisphosphate carboxylase, putative | 94% | 1.00 a | 0.30 b | 0.32 b | 0.04 c |
| Stress response | |||||||||||
| 78-4 | XP_006470782 | 8.E-12 | 67 | Citrus sinensis | 250 | Glutathione S-transferase zeta class-like isoform X1 | 100% | 1.00 a | 0.26 b | 0.24 b | 0.97 a |
| 164-1 | XP_006493708 | 3.E-04 | 47.8 | Citrus sinensis | 299 | Glutathione reductase, cytosolic-like | 88% | 1.00 a | 1.03 a | 0.14 b | 1.02 a |
| 217-2 | NP_192897 | 9.E-14 | 72.4 | Arabidopsis thaliana | 227 | Glutathione peroxidase 6 | 63% | 1.00 b | 1.23 b | 9.45 a | 1.20 b |
| 60-1 | XP_007021413 | 4.E-10 | 62.4 | Theobroma cacao | 300 | Thioredoxin M-type 4 | 71% | 1.00 b | 1.07 b | 1.15 b | 10.70 a |
| 243-1 | XP_006475833 | 1.E-38 | 141 | Citrus sinensis | 242 | 2-alkenal reductase (NADP+- dependent) -like | 97% | 1.00 b | 6.00 a | 1.06 b | 6.15 a |
| 178-1 | XP_007017815 | 5.E-05 | 48.1 | Theobroma cacao | 304 | Chaperone DnaJ-domain superfamily protein, putative | 70% | 1.00 a | 0.14 c | 0.39 b | 0.39 b |
| 83-5 | BAJ11779 | 5.E+00 | 30.8 | Corchorus tridens | 125 | Dehydration responsive protein | 78% | 1.00 c | 9.62 ab | 1.34 bc | 9.92 a |
| 219-3 | XP_007035783 | 1.E+00 | 35.4 | Theobroma cacao | 212 | Adenine nucleotide alpha hydrolases-like superfamily protein | 84% | 1.00 b | 7.91 a | 1.02 b | 5.71 a |
| 59-1 | XP_002310744 | 1.E-13 | 75.9 | Populus trichocarpa | 282 | Disease resistance family protein | 41% | 1.00 a | 0.08 b | 0.25 b | 0.09 b |
| 176-1 | XP_006494011 | 2.E-21 | 98.2 | Citrus sinensis | 286 | Putative disease resistance protein At3g14460-like | 66% | 1.00 a | 1.09 a | 0.23 b | 1.02 a |
| Autophagy and senescence | |||||||||||
| 158-1 | NP_564664 | 4.E-11 | 67.8 | Arabidopsis thaliana | 236 | Autophagy 18H-like protein | 53% | 1.00 b | 0.23 c | 2.69 a | 0.23 c |
| 2-1 | BAB33421 | 8.E-39 | 140 | Pisum sativum | 244 | Putative senescence-associated protein | 86% | 1.00 a | 1.04 a | 1.06 a | 0.12 b |
| 5-3 | BAB33421 | 1.E-25 | 104 | Pisum sativum | 191 | Putative senescence-associated protein | 89% | 1.00 a | 0.96 a | 1.02 a | 0.15 b |
| 139-8 | BAB33421 | 2.E-35 | 131 | Pisum sativum | 236 | Putative senescence-associated protein | 89% | 1.00 b | 0 c | 0 c | 3.62 a |
| 156-3 | BAB33421 | 3.E-08 | 58.2 | Pisum sativum | 274 | Putative senescence-associated protein | 69% | 1.00 a | 0.17 b | 1.06 a | 1.06 a |
| 141-7 | AAR25995 | 3.E-31 | 115 | Pyrus communis | 259 | Putative senescence-associated protein | 96% | 1.00 b | 1.01 b | 1.04 b | 7.99 a |
| 209-1 | AAR25995 | 1.E-56 | 181 | Pyrus communis | 296 | Putative senescence-associated protein | 98% | 1.00 b | 8.39 a | 1.18 b | 0.95 b |
| 217-1 | AAR25995 | 2.E-51 | 167 | Pyrus communis | 309 | Putative senescence-associated protein | 99% | 1.00 b | 3.31 a | 2.86 ab | 1.14 b |
| 219-2 | AAR25995 | 2.E-50 | 165 | Pyrus communis | 296 | Putative senescence-associated protein | 97% | 1.00 b | 1.14 b | 10.80 a | 1.11 b |
| 223-1 | AAR25995 | 2.E-49 | 162 | Pyrus communis | 314 | Putative senescence-associated protein | 97% | 1.00 a | 0.16 b | 1.09 a | 0.99 a |
| 179-6 | XP_006473584 | 1.E-37 | 138 | Citrus sinensis | 215 | Cysteine proteinase 15A-like | 98% | 0 c | 0 c | 1.00 a | 0.54 b |
| 246-9 | XP_006467009 | 2.E-21 | 94.7 | Citrus sinensis | 154 | Aspartic proteinase-like protein 1-like | 94% | 1.00 b | 1.06 b | 5.68 a | 1.13 b |
| 87-3 | XP_002882118 | 7.E-34 | 133 | Arabidopsis lyrata subsp. lyrata | 272 | Serine-type peptidase | 71% | 1.00 b | 1.16 b | 4.32 a | 1.25 b |
| 179-4 | XP_003633155 | 3.E-19 | 90.5 | Vitis vinifera | 238 | Ubiquitin carboxyl-terminal hydrolase 22-like | 93% | 1.00 a | 0.05 c | 0.06 c | 0.48 b |
| 78-2 | XP_006484457 | 1.E-29 | 117 | Citrus sinensis | 208 | Ubiquitin receptor RAD23c-like | 98% | 1.00 b | 1.16 b | 5.76 a | 1.07 b |
| Signal transduction and hormone | |||||||||||
| 19-4 | XP_003549848 | 2.E-25 | 102 | Glycine max | 274 | Putative calcium-binding protein CML19-like | 63% | 1.00 b | 0.99 b | 3.68 ab | 4.15 a |
| 19-5 | XP_003549848 | 2.E-25 | 102 | Glycine max | 274 | Putative calcium-binding protein CML19-like | 63% | 1.00 c | 1.42 bc | 4.75 a | 4.43 ab |
| 89-2 | NP_178383 | 1.E-43 | 156 | Arabidopsis thaliana | 258 | Protein kinase 2B | 88% | 1.00 a | 0.15 b | 1.11 a | 1.26 a |
| 25-3 | CAB63149 | 3.E-36 | 136 | Arabidopsis thaliana | 222 | MAP kinase | 92% | 1.00 a | 0.24 b | 1.08 a | 0.25 b |
| 140-2 | XP_006485632 | 6.E-07 | 54.7 | Citrus sinensis | 219 | Probable receptor-like protein kinase At5g47070-like isoform X1 | 96% | 1.00 a | 1.06 a | 1.08 a | 0.35 b |
| 246-3 | XP_003534233 | 7.E-03 | 42.7 | Glycine max | 221 | SRSF protein kinase 1-like isoform 1 | 71% | 1.00 a | 0.05 b | 0.05 b | 0.06 b |
| 51-15 | CAB90633 | 2.E-05 | 48.9 | Fagus sylvatica | 116 | protein phopsphatase 2C (PP2C) | 81% | 1.00 a | 0.21 b | 0.23 b | 0.24 b |
| 131-1 | XP_006350060 | 2.E-07 | 55.8 | Solanum tuberosum | 288 | Tetraspanin-8-like | 66% | 1.00 a | 0.05 b | 0.06 b | 0.05 b |
| 138-6 | NP_973890 | 1.E-11 | 67.8 | Arabidopsis thaliana | 237 | COP9 signalosome complex subunit 5a | 89% | 1.00 c | 1.80 b | 2.53 a | 0.24 d |
| 141-9 | XP_006476047 | 6.E-12 | 68.9 | Citrus sinensis | 183 | Ankyrin repeat-containing protein At3g12360-like | 79% | 1.00 a | 0.33 b | 0.35 b | 0.35 b |
| 87-7 | XP_006468682 | 2.E-25 | 107 | Citrus sinensis | 232 | WD repeat-containing protein 26-like isoform X1 | 98% | 1.00 b | 12.03 a | 1.70 b | 2.06 b |
| 178-5 | XP_006475371 | 4.E-19 | 87.8 | Citrus sinensis | 149 | IAA-amino acid hydrolase ILR1-like 4-like | 98% | 0 | 0 | 0 | + |
| Gene regulation | |||||||||||
| 188-3 | ADL36732 | 4.E-09 | 60.5 | Malus domestica | 228 | HSF domain class transcription factor | 53% | 1.00 a | 1.09 a | 0.28 b | 1.01 a |
| 23-1 | XP_006466606 | 3.E-30 | 117 | Citrus sinensis | 198 | Heat shock factor protein HSF24-like | 97% | 1.00 b | 3.36 a | 3.27 a | 3.20 a |
| 138-4 | XP_007018496 | 4.E-03 | 44.3 | Theobroma cacao | 261 | PHD finger transcription factor | 44% | 0 | 0 | + | 0 |
| 139-1 | XP_006468886 | 5.E-74 | 246 | Citrus sinensis | 375 | Putative pentatricopeptide repeat-containing protein At2g01510-like | 98% | 1.00 a | 0.57 b | 0.57 b | 1.11 a |
| 177-3 | NP_195386 | 4.E-05 | 49.7 | Arabidopsis thaliana | 235 | Pentatricopeptide repeat-containing protein | 73% | 0 | + | 0 | 0 |
| 27-4 | XP_006467029 | 7.E-27 | 112 | Citrus sinensis | 186 | DNA-directed RNA polymerase II subunit 1-like isoform X3 | 98% | 1.00 a | 0.14 b | 1.04 a | 0.97 a |
| 132-1 | XP_006479511 | 2.E-39 | 149 | Citrus sinensis | 245 | DNA repair and recombination protein RAD26-like isoform X3 | 99% | 0 | 0 | 0 | + |
| 219-4 | XP_006472001 | 3.E-15 | 76.6 | Citrus sinensis | 189 | DNA excision repair protein ERCC-1-like isoform X1 | 84% | 1.00 b | 11.60 a | 1.16 b | 1.07 b |
| 177-8 | XP_006490371 | 1.E-10 | 65.5 | Citrus sinensis | 165 | DNA mismatch repair protein MSH3-like | 97% | 1.00 a | 1.02 a | 0.22 b | 0.21 b |
| 134-13 | BAK61840 | 2.E-16 | 82.8 | Citrus unshiu | 182 | Gag-pol polyprotein | 70% | 1.00 b | 0.04 c | 0.04 c | 3.25 a |
| 134-4 | XP_003614387 | 3.E-14 | 77.4 | Medicago truncatula | 279 | RRNA intron-encoded homing endonuclease | 93% | + | 0 | 0 | 0 |
| 246-2 | XP_003614387 | 1.E-08 | 60.5 | Medicago truncatula | 234 | RRNA intron-encoded homing endonuclease | 86% | 1.00 b | 10.50 a | 1.39 b | 1.05 b |
| 83-2 | XP_003614389 | 3.E-22 | 97.1 | Medicago truncatula | 210 | RRNA intron-encoded homing endonuclease | 87% | 1.00 a | 0.13 b | 1.13 a | 0.12 b |
| 162-5 | XP_006473637 | 3.E-07 | 55.1 | Citrus sinensis | 149 | 5'-3' exoribonuclease 3-like isoform X2 | 96% | 0 | + | 0 | 0 |
| 246-5 | XP_006472153 | 9.E-24 | 100 | Citrus sinensis | 169 | Pre-mRNA-splicing factor 38A-like | 94% | 1.00 b | 1.09 b | 10.40 a | 1.09 b |
| Cell transport | |||||||||||
| 175-7 | XP_006489422. | 3.E-06 | 49.7 | Citrus sinensis | 136 | Non-specific lipid-transfer protein-like protein At2g13820-like | 96% | 0 c | 0 c | 1.00 a | 0.38 b |
| 59-3 | XP_007026766 | 7.E+00 | 33.9 | Theobroma cacao | 243 | Major facilitator superfamily protein, putative | 32% | 0 c | 0 c | 1.00 a | 0.23 b |
| 252-1 | XP_006464865 | 4.E-34 | 126 | Citrus sinensis | 275 | Citrate-binding protein-like | 77% | 0 c | 1.00 a | 0 c | 0.35 b |
| 141-8 | XP_006473247 | 3.E-31 | 123 | Citrus sinensis | 209 | Patellin-2-like | 84% | 0 | 0 | 0 | + |
| 177-6 | XP_007012650 | 2.E-03 | 43.5 | Theobroma cacao | 202 | Membrane lipoprotein | 82% | 0 | 0 | 0 | + |
| 134-5 | XP_006469059 | 2.E-35 | 130 | Citrus sinensis | 260 | Ras-related protein RABA1f-like | 95% | 1.00 b | 3.37 a | 0.16 c | 2.79 a |
| 19-3 | XP_006467607 | 3.E-57 | 194 | Citrus sinensis | 316 | Protein transport protein Sec61 subunit alpha-like | 94% | 1.00 b | 7.14 a | 6.69 a | 6.77 a |
| 180-2 | XP_006480618 | 2.E-11 | 65.9 | Citrus sinensis | 240 | Syntaxin-71-like | 79% | 1.00 a | 0.06 b | 1.14 a | 0.05 b |
| 162-4 | XP_006487552 | 1.E-15 | 80.1 | Citrus sinensis | 177 | ADP-ribosylation factor GTPase-activating protein AGD3-like | 100% | 0 | + | 0 | 0 |
| 78-3 | XP_006483372 | 3.E-11 | 66.2 | Citrus sinensis | 147 | Putative clathrin assembly protein At2g25430-like | 97% | 1.00 a | 1.02 a | 1.06 a | 0.10 b |
| 136-8 | XP_006472885 | 2.E-29 | 117 | Citrus sinensis | 200 | Target of Myb protein 1-like isoform X1 | 92% | 0 | 0 | + | 0 |
| 87-5 | AFX72760 | 5.E-32 | 124 | Litchi chinensis | 236 | ATP/ADP carrier protein, partial | 97% | 1.00 a | 0.16 b | 0.10 b | 0.13 b |
| Cell wall modification | |||||||||||
| 124-1 | XP_006480190 | 1.E-12 | 70.1 | Citrus sinensis | 149 | Probable pectate lyase 8-like | 94% | 1.00 a | 0.08 b | 1.03 a | 0.07 b |
| 17-1 | XP_006493306 | 2.E-44 | 160 | Citrus sinensis | 257 | Probable pectinesterase/pectinesterase inhibitor 61-like | 97% | 0 | + | 0 | 0 |
| 51-1 | XP_007042653 | 4.E-25 | 107 | Theobroma cacao | 241 | Glycosyl hydrolase family 10 protein, putative | 65% | 1.00 b | 0.11 c | 0.09 c | 1.64 a |
| 148-1 | XP_006469451 | 6.E-01 | 36.6 | Citrus sinensis | 189 | Fasciclin-like arabinogalactan protein 2-like | 90% | 1.00 a | 1.08 a | 0.17 b | 0.20 b |
| Others (unknown/unclassified) | |||||||||||
| 204-2 | XP_003588355 | 1.E-04 | 48.1 | Medicago truncatula | 170 | Mitochondrial protein, putative | 88% | 1.00 a | 0.59 b | 1.09 a | 0.57 b |
| 149-1 | XP_006480893 | 7.E-43 | 155 | Citrus sinensis | 276 | Uncharacterized protein LOC102616798 | 70% | 0 | 0 | + | 0 |
| 159-2 | XP_006468400. | 2.E-13 | 73.2 | Citrus sinensis | 219 | Uncharacterized protein LOC102609810 | 78% | 0 b | 1.00 a | 0 b | 0.99 a |
| 179-1 | NP_001169009 | 2.E-23 | 98.6 | Zea mays | 318 | Hypothetical protein | 74% | 0 | 0 | 0 | + |
| 180-5 | XP_006421131 | 8.E-18 | 84.3 | Citrus clementina | 260 | Hypothetical protein CICLE_v10005475mg | 87% | 1.00 a | 0.06 b | 0.07 b | 0.07 b |
| 187-2 | XP_006492168 | 2.E-06 | 54.7 | Citrus sinensis | 292 | Uncharacterized protein LOC102628400 | 87% | 1.00 c | 4.51 b | 1.23 c | 9.38 a |
| 19-2 | NP_189331 | 7.E-18 | 85.9 | Arabidopsis thaliana | 206 | Uncharacterized protein | 82% | 1.00 a | 0.15 b | 0.18 b | 0.16 b |
| 237-1 | XP_006478809 | 2.E-46 | 163 | Citrus sinensis | 263 | Uncharacterized protein LOC102629577 | 100% | 1.00 b | 5.24 a | 1.19 b | 5.21 a |
| 249-1 | XP_004499954 | 2.E-37 | 131 | Cicer arietinum | 305 | Uncharacterized protein LOC101515437 | 96% | 1.00 b | 5.93 a | 6.66 a | 1.01 b |
| 87-4 | XP_006444509 | 4.E-14 | 74.3 | Citrus clementina | 249 | hypothetical protein CICLE_v10021318mg | 97% | 1.00 b | 0.92 b | 11.3 a | 11.9 a |
| 204-1 | XP_003608262 | 1.E-09 | 60.5 | Medicago truncatula | 252 | hypothetical protein MTR_4g091430 | 76% | 1.00 a | 1.14 a | 0.57 b | 1.16 a |
Note. 2.5 B - Al: 2.5 μM B + 0 mM Al; 2.5 B + Al: 2.5 μM B + 1.2 mM Al; 20 B - Al: 20 μM B + 0 mM Al; 20 B + Al: 20 μM B + 1.2 mM Al.Ratio means the ratio of different treatments to control (set as 1). Usually, the control was 2.5 B - Al. If TDF was not detected in the treatment, the control would be 2.5 B + Al and so on.
0 means TDF was not detected in the treatment; + means TDF was detected only in the treatment.
Values are means of 3 replicates.
Differences among four treatments were analyzed by two (B) × two (Al) ANOVA.
Within a row, values followed by different letters indicate a significant difference at P < 0.05.