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. 2015 Mar 6;11(3):e1005036. doi: 10.1371/journal.pgen.1005036

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© 2015 Orgil et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 5 — A. Strains YOG3040 (wpl1Δ SCC2-3V5), YOG3002 (wpl1Δ SCC2-3V5 SCC3-6HA) or YOG3003 (wpl1Δ SCC2-3V5 scc3-I358ins-6HA) were grown to the mid-log phase in YEPD media. Cells were lysed and subjected to immunoprecipitation against the HA tag of Scc3. The co-precipitation of Scc2 was detected by anti V5 antibody. B. Strain YOG3050 (PDS5-3V5-AID SCC2-12MYC) YOG3044 (PDS5-3V5-AID SCC2-12MYC SCC3-6HA) and YOG3045 (PDS5-3V5-AID SCC2-12MYC scc3-6HAI358ins-6HA) were grown to the mid-log phase. Cells were divided into two flasks and 1 mM IAA was added to one of them for 60 minutes. The depletion of Pds5-3V5 was measured in the extract by Western blot with antibodies against V5. Cells were used for immunoprecipitation of Scc3 with anti-HA antibodies. The co-precipitation of Scc2 was detected by anti MYC antibody.