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. 2015 Mar 6;11(3):e1005036. doi: 10.1371/journal.pgen.1005036

Fig 6. Scc3 does not affect the Smc3-Mcd1 interaction.

Fig 6

A. Strain YOG3049 (SCC3-AID-V5 SMC3-6HA) was grown to the mid-log phase. Cells were divided into two flasks and 1 mM IAA was added to one of them for 60 minutes. The depletion of Scc3-3V5 was measured in the extract by Western blot with antibodies against V5. Un-depleted and depleted Scc3 cells were used for immunoprecipitation of Smc3-6HA with anti-HA antibodies. The co-precipitation of Mcd1 was detected by anti-Mcd1 antibody. B. Strain YOG3027 (SCC3-AID-V5 SCC2-12MYC) was grown to the mid-log phase. Cells were divided into two flasks and 1 mM IAA was added to one of them for 120 minutes. The depletion of Scc3-3V5 was measured in the extract by Western blot with antibodies against V5. Cells were used for immunoprecipitation of Scc2-12Myc with anti-MYC antibodies. The co-precipitation of Mcd1 was detected by anti-Mcd1 antibody.