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. 2015 Mar 6;11(3):e1005036. doi: 10.1371/journal.pgen.1005036

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© 2015 Orgil et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 7 — A. Strain YIO81 (scc3-6), carrying an ectopic copy of SCC3-6HA (YOG3021), scc3-F367A-6HA (YOG3022), scc3-R370A-6HA (YOG3023), scc3-Y371A-6HA (YOG3024), scc3-K372A-6HA (YOG3025) or scc3-D373A-6HA (YOG3026) were grown to saturation in YPD media. Tenfold serial dilution of the strains were plated on YPD plates and grown at either permissive (23°C) or restrictive (35°C) temperature. B. Strains YIO81 (scc3-6), YOG3021 (SCC3-6HA scc3-6), YOG3024 (scc3-Y371A-6HA scc3-6), YOG3025 (scc3-K372A-6HA scc3-6) and YOG3026 (scc3-D373A-6HA scc3-6) were analyzed for sister chromatid cohesion using the GFP spot assay (n = 3). Cell cycle progression was determined by flow cytometry analysis (S4 Fig). C. Co-immunoprecipitation of Mcd1 with Scc3-6HA, scc3-Y371A-6HA, scc3-K372A-6HA or scc3-D373A-6HA. Precipitation of Scc3 was done by using an anti-HA antibody.